1. Smoke constituents viz., Tar, Nicotine and Carbon monoxide

2. Solanesol by HPLC

3. Nicotine, Reducing sugars and Chlorides by Auto-analyzer

4. Nitrogen, Phosphorus, Potassium, Calcium, Magnesium, Sulphur, Silica and Ash in leaf/ soil/ fertilizer

5. Leaf physical quality parameters viz., Filling Value (FV) and Equilibrium Moisture Content (EMC)

6. Micronutrients viz., Zinc, Copper, Iron, Manganese & Boron in leaf/ soil/ fertilizer by AAS

7. Soil properties and fertility -- pH, Electrical conductivity, Chlorides, Organic carbon, Available P & K

8. Water quality -- pH, Electrical conductivity, Chlorides